DNA
Part:BBa_K2715023:Design
Designed by: Daniel Partridge Group: iGEM18_Nottingham (2018-10-08)
C.difficile Toxin B promoter 5' UTR without RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 66
Design Notes
The translation initiation site was predicted using the BPROM algorithm (see references below), and the 5' UTR region identified.
Source
This basic part is the 5' UTR portion of the tcdB toxin gene regulatory region from the Gram-positive organism Clostridium difficile.
References
V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78