DNA

Part:BBa_K2715023:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


C.difficile Toxin B promoter 5' UTR without RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 66


Design Notes

The translation initiation site was predicted using the BPROM algorithm (see references below), and the 5' UTR region identified.

Source

This basic part is the 5' UTR portion of the tcdB toxin gene regulatory region from the Gram-positive organism Clostridium difficile.

References

V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78